Repbase Reports

2008, Volume 8, Issue 7
July 31, 2008
Copyright © 2001-2016 - Genetic Information Research Institute
ISSN# 1534-830X
Page 750

EnSpm-2_DR

EnSpm-2_DR is an autonomous DNA transposon - a consensus.

Submitted:
31-Jul-2008
Accepted:
31-Jul-2008
Key Words:
EnSpm; DNA transposon; Transposable Element; Autonomous DNA transposon; EnSpm-2_DR
Source:
Danio rerio
Organism:
Danio rerio
Taxonomy:
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Cyprinidae; Danio
[1] Authors:
Kapitonov,V.V. and Jurka,J.
Title:
Zebrafish En/Spm DNA transposons.
Journal:
Repbase Reports 8(7), 750-750 (2008)
Abstract:
EnSpm-2_DR is young family of autonomous En/Spm DNA transposons. The consensus sequence was derived based on multiple alignment of several copies of EnSpm-2_DR that are less then 5% divergent from each other. The consensus sequence codes for a 815-aa En/Spm transposase. Based on multiple alignment of diverse En/Spm tranposases from plants, fungi and animals, we suggest that the En/Spm catalytic core is defined by a DDE triad. Its coordianates in EnSpm-2_DR transposase are defined by the 380-469-634 aa positions. Zebrafish EnSpm tranpsosase contains also in its N-terminal portion a novel type of zinc finger motif, called ENS-finger: C-x2-C-x12-H-x4-H-x11-C-x4-C-x12-H-x4-H. EnSpm-2_DR transposons are charecterized by 2-bp target-site duplications (TA - most frequent, CA, TG) and imperfect 30-bp terminal inverted repeats (6 mismatches). EnSpm-2_DR is a composite transposon: its 869-1079 portion is similar to the 172-388 portion of the TDR15 transposon. Contrary to plant En/Spm transposons with the canonical CACTA 5'-termini, zebrafish En/Spm transposons contain the CACAG or CACTG 5'-termini. Zebrafish En/Spm transposons differ also from their plant relatives by a target-site duplication length: it is 2-bp in zebrafish (3-bp in plants).
Derived:
[1] (Consensus)
Download Sequence - Format:
IG, EMBL, FASTA
References:

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