Repbase Reports |
|---|
| 2001, Volume 1, Issue 4 |
| April 30, 2001 |
| Copyright © 2001-2016 - Genetic Information Research Institute |
| ISSN# 1534-830X |
| Page 31 |
VANDAL20 |
|||
|---|---|---|---|
Autonomous DNA transposon VANDAL20, a fossil. |
|||
|
Submitted: 30-Apr-2001 |
Accepted: 30-Apr-2001 |
||
|
Key Words: Autonomous DNA transposon; MuDR superfamily; MuDR transposase; SUMO protease; VANDAL20 |
|||
|
Source: thale cress |
Organism: Arabidopsis thaliana |
Taxonomy: Eukaryota; Viridiplantae; Charophyta/Embryophyta group; Embryophyta; Tracheophyta; euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; Rosidae; Capparales; Brassicaceae; Arabidopsis |
|
| [1] |
Authors: Kapitonov,V.V. and Jurka,J. |
||
|
Title: VANDAL20, a family of monstrous MuDR-like DNA transposons. |
|||
|
Journal: Repbase Reports 1:(4) p.31 (2001) |
|||
Abstract: VANDAL20 is a fossil of a MuDR-like autonomous DNA transposon. It has been active transpositionally relatively recently; its exon/intron structure is well preserved, and it is flanked by a perfect 9-bp target site duplication (TTAATTAAG). VANDAL20 has imperfect subterminal 52-bp inverted repeats (8 mismatches, positions 17-68 and 12704-12653). A 8790aa MuDR-like transposase, VANDAL20p1, is encoded by 4 exons (predicted by FGENESH and GENSCAN). CDS join(610..616,709..2656,2811..3405,3479..3568) VANDAL20p1: MEVDDIVVVQAGRWILYSTGEWEFKLDSCRHGRAMSLMGLRTLRDLELKVRHIFGLEKPS ISLELSYIFEDRLALLAGVLPGPTMIGSDWQFRCFKSLSIGDKSVNLFVCFREGDSVIVG PACEPRQTSASVVKYFEAGEPTFAGIVQFRDKPVPAVGGGYFAGLLSSVQDNMDAHPPNN HSDFVQSTTVCDLSSQWDISDELLLEALEAVEKSGAFVTAAGVDTSLPAEVPDSGDDSES LAESDESSSLSTGSDLEVSEFDDDTLHENTLQSDKEIVVHEDIGDDDFIEQPPKKKVLLQ NTNPGAMTEFLETCQKGISIDQVYGYEDLEPMFGDDDIDAKAAYVDLTKEDDNMFVGRTF ASREDFRIALSIYAINRIFRFKFTRYEKHYLVAECYDKKNCDWRVRAHQVGGDSEEYEVR LAKLEHVCKVQTRSRFSKHATSKVVAALLRAKYAKAFCGPRARDLPDSLLREHNVRMTYW KCWKAKELAVETAQGTDESSFSLLPVYLHVLQLANPGTVYHLETELDDIGDDRFKYVFLS LGASVKRLKYIRRVVVVDGTHLFGKYLGCLLTASCQDANFQIFPIAFAVVDSETDHSWTW FMNKLSEIIKDGPDLTFVSDRNQSIFKSVGLVFPQAHHGACLVHIRRNVKGRVHFTGERY NLMSSNIAESLNNALLPARGSPVVALLEFIRKMLARWFESRRKKISRTVGDIPIAVEREL MKRFKGALGMSVSAVGSWDFEVVAKDGEQFHVSLEQQSCSCLEFQAIRIPCTHAMAACHD RGLEFRTLVGEMHRLPMWSPTVQAPILLVRDPSEVKVPEEIRSLCLMPPKTKRPAGRPPK LRIHSVGEYEGNNKSKTPNKGGRCGGLGHNRVTCKTPLG A ~224-aa protein of unknown function is encoded by one or two exons in a region 4794-5464. X masks a short deletion reconstructed based on sequences of VANDAL20p2-like proteins encoded by different VANDALs. VANDAL20p2: MPDIPDDLLAKIVKLVADDRWWLLGPILKAGRRGRDMVYRDNVLKDACIYSLCSDPSDLY YFRNQDTDVVHQGRYRPFFERCLKAGNKTAIYYEGLRVVAEGCDLNGGIELLAQLVPEDG XGWRSMCLGNEAMAVHYLEQFGIFHAPLSTQRVRLWGEELVRDLRPYRRISHASYRKAFI YPLCKGIPSPDCAMDCALASSGYENFCNDCYLWWLSRKVCQMV VANDAL20p2 is 25% identical (blastp, E=3e-11) to VANDAL20p3, a 201-aa protein encoded by the second strand. CDS complement(join(6864..7457,7570..7581)) VANDAL20p3: MSLVKLGDDDAYYLAPVILTNKRGRDIALSRDIVKSANLTAFCLEPPKIYPGGVARSFFT RQALYYEALRLVTRERDIQGSLALLNELVPEDESATVAYAMFQMCAERGDLAGATFDMIF ESLGDRLLFNIYSEDLEAECEGLIDTLLAYEPANEDTFGPIWEFAYSKRINVPDCAYWHE GEHYCEGCYIFNAALRICEI VANDAL20's second strand encoded also a 958-aa protein called VANDAL20p4. CDS complement(join(8459..9082,9169..9252,9331..9585, 9664..9831,10128..10388,10477..10959,11102..11461, 11629..12033,12144..12269,12368..12466)) VANDAL20p4 (958 aa): MTKKKSSKSKTISPPVVMKQEPKRRNSRADAQRDTHAKSPAKSPASARGLRDESPISPAP AKIPAKSGGLRNESKQGDERCRDSEFTNPSLPCRLLHPSEFPDGGRLNIHSKPEYLGTLV ELLEGSEAWDELIASQFKKLFELPVARCHHSAKLIHGMLTRQLLTKNKHELWTVYGGFPL RFSIREFQMTTGLICDKLPSESKVEDYHDPAYLNRKQHRVTRDFVEMLHDIEFFLSYPWG RVSFESTMERFGPSAGEEDPIAELKARLSQKSSVCYGFPLALQLLVLKSVPALCSRIKDT VDFRNFIQKLFVDLGRTILLRESDVLDAEQTANLSVEYTLYQSETVKGDLSWPDEKEDEG VELIISLIKQKHHFKAETWPIHSIQCSSKDAPAADAEYVGVALDTDDVEFVGTRTVGPED KCEPSKAVKRAKKEYYPIRRKPFTRSSKKEGLNVEPLPTQDKAATPVGKTVHKKTAKQDA TLIKQAPLLSLLLMEKATETAAGAPETPKSKSKKKKETKQPVTRKRKREEVTSLSSGSSS SGLNHGPAVDTDIGVDGGGTVNLEGSVDPWELYGSTKNISEPQEMGRESPTSPVPTATQD KPMDTKPIKTMEEPLDLGNRAGSPRQTPCSPVTPIAQVIPDSSIVAVSPAYFEKERTNIR QPVPGKRVPIRSCFLDTTKYSVDPRISALFKSRTKEPAYTPLPKLDDGEFQTFKRLLEDD PQSEYTICTGHTVTNNHFLDIATKETWVNTEHMQLITGMLMKRRGKAYSQRRVAIIDTWF AMLLSTHYEDFQKCEDKAKYEWGKTIKGYITGKSTGIHMKSEFLKHVDVVYVPMNWGCSH WVGLVINLKQQSIDVLDSFISPTPEEAVEFQMTPIVSSCPTLTTEMTTEPFSCTRVTGLY DNKGDGDCGPVACKFLEMHAHGMDYTDMTALTDEVVIRIQKVYAMDTYAAFVEWEDEY C-terminal portion of VANDAL20p4 is similar to the SUMO-like protease, which is present in highly divergent forms in different VANDAL-like families. These proteases are functional elements of VANDALs that help them to multiply.
|
|||
|
Derived: Positions 104443 91724 Accession No AL161499 GenBank (rel. 126.0) |
|||
|
Download Sequence - Format: IG, EMBL, FASTA |
|||
References:
|
|||